Post-mortem brain analyses of the Lothian Birth Cohort 1936: Extending lifetime cognitive and brain phenotyping to the level of the synapse: Data set from publication Henstridge et al 2015 Acta Neuropath Comms
Date Available
2016-06-01Type
datasetData Creator
Henstridge, Christopher MJackson, Rosemary J
Kim, JeeSoo M
Herrmann, Abigail G
Wright, Ann K
Harris, Sarah
Bastin, Mark E
Starr, John M
Wardlaw, Joanna
Gillingwater, Thomas H
Smith, Colin
McKenzie, Chris-Anne
Cox, Simon R
Deary, Ian J
Spires-Jones, Tara L
Publisher
University of Edinburgh. School of Biomedical SciencesRelation (Is Referenced By)
https://doi.org/10.1186/s40478-015-0232-0Metadata
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Citation
Christopher M. Henstridge; Rosemary J. Jackson; JeeSoo M. Kim; Abigail G. Herrmann; Ann K. Wright; Sarah Harris; Mark E. Bastin; John M. Starr; Joanna Wardlaw; Thomas H. Gillingwater; Colin Smith; Chris-Anne McKenzie; Simon R. Cox; Ian J. Deary; Tara L. Spires-Jones. (2016). Post-mortem brain analyses of the Lothian Birth Cohort 1936: Extending lifetime cognitive and brain phenotyping to the level of the synapse: Data set from publication Henstridge et al 2015 Acta Neuropath Comms, 1936-2015 [dataset]. University of Edinburgh. School of Biomedical Sciences. https://doi.org/10.7488/ds/1414.Description
Non-pathological, age-related cognitive decline varies markedly between individuals and places significant financial and emotional strain on people, their families and society as a whole. Understanding the differential age-related decline in brain function is critical not only for the development of therapeutics to prolong cognitive health into old age, but also to gain insight into pathological ageing such as Alzheimer’s disease. The Lothian Birth Cohort of 1936 (LBC1936) comprises a rare group of people for whom there are childhood cognitive test scores and longitudinal cognitive data during older age, detailed structural brain MRI, genome-wide genotyping, and a multitude of other biological, psycho-social, and epidemiological data. Synaptic integrity is a strong indicator of cognitive health in the human brain; however, until recently, it was prohibitively difficult to perform detailed analyses of synaptic and axonal structure in human tissue sections. We have adapted a novel method of tissue preparation at autopsy to allow the study of human synapses from the LBC1936 cohort in unprecedented morphological and molecular detail, using the high-resolution imaging techniques of array tomography and electron microscopy. This allows us to analyze the brain at sub-micron resolution to assess density, protein composition and health of synapses. Here we present data from the first donated LBC1936 brain and compare our findings to Alzheimer’s diseased tissue to highlight the differences between healthy and pathological brain ageing. Our data indicates that compared to an Alzheimer’s disease patient, the cognitively normal LBC1936 participant had a remarkable degree of preservation of synaptic structures. However, morphological and molecular markers of degeneration in areas of the brain associated with cognition (prefrontal cortex, anterior cingulate cortex, and superior temporal gyrus) were observed. Our novel post-mortem protocol facilitates high- resolution neuropathological analysis of the well-characterized LBC1936 cohort, extending phenotyping beyond cognition and in vivo imaging to now include neuropathological changes, at the level of single synapses. This approach offers an unprecedented opportunity to study synaptic and axonal integrity during ageing and how it contributes to differences in age-related cognitive change.The following licence files are associated with this item: