General calibration of microbial growth in microplate readers: Data supporting Figure 4
Date Available
2016-07-21Type
datasetData Creator
Pilizota, TeutaStevenson, Keiran
Clark, Ivan
Publisher
University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems BiologyRelation (Is Referenced By)
https://doi.org/10.1101/061861Metadata
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Citation
Pilizota, Teuta; Stevenson, Keiran; Clark, Ivan. (2016). General calibration of microbial growth in microplate readers: Data supporting Figure 4, [dataset]. University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology. https://doi.org/10.7488/ds/1447.Description
Data supporting figure 4 of the paper (Stevenson et al, https://doi.org/10.1101/061861). Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from antibiotic efficacy studies, studies of growth under different nutritional or stress environments, to studies of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.The following licence files are associated with this item: