Small interfering RNAs (siRNA) genome-scale screen data for investigating host factors (HFs) involved in Herpes Simplex Virus Type 1 Replication
Date Available
2016-08-02Type
datasetData Creator
Griffiths, SamanthaKoegl, Manfred
Boutell, Chris
Zenner, Helen
Crump, Colin
Pica, Francesca
Gonzalez, Orland
Friedel, Caroline
Barry, Gerald
Martin, Kim
Craigon, Marie
Chen, Rui
Kaza, Lakshmi
Fossum, Even
Fazakerley, John
Efstathiou, Stacey
Volpi, Antonio
Zimmer, Ralf
Ghazal, Peter
Haas, Jurgen
Publisher
University of Edinburgh. Medical school. Division of infection & Pathway MedicineRelation (Is Referenced By)
http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003514Metadata
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Citation
Griffiths, Samantha; Koegl, Manfred; Boutell, Chris; Zenner, Helen; Crump, Colin; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline; Barry, Gerald; Martin, Kim; Craigon, Marie; Chen, Rui; Kaza, Lakshmi; Fossum, Even; Fazakerley, John; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jurgen. (2016). Small interfering RNAs (siRNA) genome-scale screen data for investigating host factors (HFs) involved in Herpes Simplex Virus Type 1 Replication, 2012-2013 [dataset]. University of Edinburgh. Medical school. Division of infection & Pathway Medicine. https://doi.org/10.7488/ds/1451.Description
Small interfering RNA (siRNA) data associated with the following manuscript by Griffiths et al.(2013): A Systematic Analysis of Host Factors Reveals a Med23-Interferon-l Regulatory Axis against Herpes Simplex Virus Type 1 Replication. Herpes simplex virus type 1 (HSV-1) is a neurotropic virus causing vesicular oral or genital skin lesions, meningitis and other diseases particularly harmful in immunocompromised individuals. To comprehensively investigate the complex interaction between HSV-1 and its host we combined two genome-scale screens for host factors (HFs) involved in virus replication. A yeast two-hybrid screen for protein interactions and a RNA interference (RNAi) screen with a druggable genome small interfering RNA (siRNA) library confirmed existing and identified novel HFs which functionally influence HSV-1 infection. Hela cells were reverse-transfected with siRNA SMARTpools (4 siRNAs per gene). After 48 h the siRNAs were tested for cytotoxicity (3 replicates) or the capacity to influence replication of the HSV-1 GFP reporter virus C12 (6 replicates) from 24 to 80 h post-infection. Virus replication slopes during the linear phase were calculated and normalized to mock-transfected cells. Replication slopes were then compared to replication upon knockdown of essential (ICP4, VP16) or non-essential (VP11/12) viral genes, a cellular receptor for HSV-1 (HVEM) or control RISC-free siRNA (RSCF).The following licence files are associated with this item: