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Depositordc.contributorJohnson, Craig
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherZoetis Inc.en_UK
Spatial Coveragedc.coverage.spatialUKen
Spatial Coveragedc.coverage.spatialUNITED KINGDOMen
Data Creatordc.creatorJohnson, Craig
Data Creatordc.creatorClements, Dylan
Date Accessioneddc.date.accessioned2016-08-04T11:24:42Z
Date Availabledc.date.available2017-07-26T04:15:20Z
Citationdc.identifier.citationJohnson, Craig; Clements, Dylan. (2017). Cytokine stimulation of canine chondrocytes and osteoblasts, [dataset]. University of Edinburgh. College of Medicine and Veterinary Medicine. Roslin Institute. https://doi.org/10.7488/ds/1455.en
Persistent Identifierdc.identifier.urihttps://hdl.handle.net/10283/2073
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/1455
Dataset Description (abstract)dc.description.abstractIn vitro modelling of osteoarthritis typically uses cytokines to induce a catabolic phenotype in joint cells. This model was used to identify the response to a cytokine parameter identified as most representative of early stage osteoarthritis. Canine cells were used, isolated from the humeral head of post-mortem subjects. The cartilage cells were stimulated with 5 ng/ml of IL8 for 2 hours, whilst the osteoblasts were stimulated with 500 pg/mL of IL1 and 500 pg/mL of IL8. After the indicated culture time, RNA was extracted and a microarray was performed to identify gene expression changes.en_UK
Dataset Description (TOC)dc.description.tableofcontentsFiles are .CEL format produced during Affymetrix Microarray Experiments. It is a widely used format for these experiments. A number of software packages are capable of opening these files, including Affymetrix Expression Console and Partek Genomics Suite.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. College of Medicine and Veterinary Medicine. Roslin Instituteen_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subject Classificationdc.subject.classificationVeterinary Medicineen_UK
Titledc.titleCytokine stimulation of canine chondrocytes and osteoblastsen_UK
Alternative Titledc.title.alternativeJoint Cells Stimulated with Cytokines (As in abstract)en_UK
Typedc.typedataseten_UK

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