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Depositordc.contributorRibchester, Richard R.
Depositordc.contributorWard, Paulineen
Funderdc.contributor.otherMRC - Medical Research Council
Funderdc.contributor.otherNorth British Hotels Trust
Funderdc.contributor.otherMotor Neurone Disease Association
Data Creatordc.creatorHirst, Theodore C.
Data Creatordc.creatorRibchester, Richard R.
Citationdc.identifier.citationHirst, Theodore C.; Ribchester, Richard R. (2017). Segmentation of the 4DL Connectome 2: YFP-H 1.3-19.3, [dataset]. University of Edinburgh. Centre for Integrative Physiology.
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Dataset Description (abstract)dc.description.abstractConnectomic analysis of the nervous system aims to discover and establish principles that underpin normal and abnormal neural connectivity and function. In Hirst et al (2013) we performed image analysis of motor unit connectivity in the fourth deep lumbrical muscle (4DL) of mice, using transgenic expression of fluorescent protein in motor neurones as a morphological reporter. We developed a method that accelerated segmentation of confocal image projections of 4DL motor units, by applying high resolution (63×, 1.4 NA objective) imaging or deconvolution only where either proved necessary, in order to resolve axon crossings that produced ambiguities in the correct assignment of axon terminals to identified motor units imaged at lower optical resolution (40×, 1.3 NA). This dataset is the outcome of Yellow Fluorescent Protein H (YFP-H) expression in numerous muscles of the mouse footpad. The zip archive contains subdirectories numbered according to the muscles ("1.3-3L", "8.6-2", "14.6-2" etc). The remainder of the data relating to this study can be found grouped together in the "Connectomic analysis of the mouse fourth deep lumbrical muscle connectome" DataShare Collection at
Publisherdc.publisherUniversity of Edinburgh. Centre for Integrative Physiology
Relation (Is Referenced By)dc.relation.isreferencedby
Relation (Is Referenced By)dc.relation.isreferencedbyHirst, TC; Ribchester, RR (2013)"Segmentation of the mouse fourth deep lumbrical muscle connectome reveals concentric organisation of motor units" J. Physiol, DOI: 10.1113/jphysiol.2013.258087
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectneuromuscular junction
Subjectdc.subjectconnectomic analysis
Subjectdc.subjectmotor unit
Subjectdc.subjectconfocal microscopy
Subjectdc.subjectmotor nerve
Subjectdc.subjectfluorescent protein
Subjectdc.subjectmotor unit size
Subject Classificationdc.subject.classificationSubjects allied to Medicine::Neuroscience
Titledc.titleSegmentation of the 4DL Connectome 2: YFP-H 1.3-19.3

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