Wang Lab
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Our interests include both fundamental and applied synthetic biology with focus on three synergistic research themes, i.e. the foundational technology, healthcare and industrial biotechnology applications. We use a bottom-up engineering approach to design synthetic biological circuits and systems using characterised/standardised biological parts and devices. We build novel customised gene circuits for sensing, advanced information processing and computing of multiple cellular and environmental signals with applications ranging from biosensing, biocomputing and biomanufacturing to biotherapy.
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Orthogonality and burden of a heterologous AND gate gene circuit in E. coli host
RNA-Seq sample preparation and sequencing E. coli NCM3722 cultures were grown and growth stopped 4 hrs post day dilution by adding 1/10 volume of 5% phenol 95% ethanol (v/v). Cells were harvested by centrifugation (4500 ...