Data in support of 'Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors'
Date Available
2017-12-31Type
datasetData Creator
Rosser, SusanKleinjan, Dirk-Jan
Publisher
University of Edinburgh, UK Centre for Mammalian Synthetic Biology, School of Biological SciencesMetadata
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Citation
Rosser, Susan; Kleinjan, Dirk-Jan. (2017). Data in support of 'Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors', [dataset]. University of Edinburgh, UK Centre for Mammalian Synthetic Biology, School of Biological Sciences. https://doi.org/10.7488/ds/2121.Description
This dataset contains a file with images of the full Western blots used in the manuscript "Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors", plus a file with DNA sequences of constructs used in the study. The nuclease-deactivated variant of CRISPR/Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9/Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.The following licence files are associated with this item: