Functional Genetics and Development
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The Division of Functional Genetics and Development investigates the mechanisms and control of animal growth and development, from the molecular to the whole organism level. The research of Functional Genetics and Development contributes to advances in frontier bioscience underpinning both human and animal health and to understanding the development and growth of model and farm animal species.
Image: Detail from Fig.4 of Jones et al. (2017). This is a heatmap of proteomic data from the dataset: Wishart, Thomas. (2017). "TMT generated proteomic identification and quantification of muscle and NMJ enriched fractions in mice and humans", [dataset]. https://doi.org/10.7488/ds/2240.
Items in this Collection
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Synaptic and non synaptic proteome in ageing murine cortical isolates
Label free proteomic analysis of "synaptic" and "non-synaptic" mitochondrial subpopulations isolated from murine cortex throughout the process of "normal healthy" ageing. These are sub populations of mitochondria as isolated ... -
Proteomic correlates of vulnerability in the presymptomatic synapse of a mouse model of SMA and a rat model of ALS Type 8
Data Generation: Tandem-mass-tagged mass-spectrometry (TMT-MS/MS) analyses of hippocampal synaptosomes of the Taiwanese mouse model of SMA, generated from n=5 each wildtype (Smn+/+), heterozygous (Smn+/-; SMN2tg/0) and ... -
Proteomic analysis to identify the pathways and processes affected by altered levels of Sideroflexin3 (SFXN3) in the synapse.
Proteomic data from TMT based analysis of synapses biochemically isolated from Sfxn3-KO mice (http://www.mousephenotype.org/data/genes/MGI:2137679). See attached word/.txt description for further information. Briefly ... -
Supplementary files supporting the paper "Microarray profiling emphasizes transcriptomic differences between hippocampal in vivo tissue and in vitro cultures"
Differential expression was performed using the R package "limma" (R package version 3.44.3) (Supplementary files 1-4; 1_diff_expr_WT_cell_vs_tissue, 2_diff_expr_101LL_cell_vs_tissue, 3_diff_expr_cell_WT_ vs_101LL, ... -
Microarray data CEL files supporting the paper "Microarray profiling emphasizes transcriptomic differences between hippocampal in vivo tissue and in vitro cultures"
Affymetrix microarray processing produced 16 (4 WT cell, 4 101LL cell, 4 WT tissue, 4 101LL tissue) probe cell intensity data (CEL) files. Primary hippocampal cell cultures are routinely used as an experimentally accessible ... -
lentiviral mediated apoE4 transduction in primary neuronal culture
To identify the molecular changes in signalling pathways triggered by altered apoE4 expression, we used lentiviral transduction to overexpress human apoE4 in 18-21 DIV primary hippocampal neurons. The molecular consequences ... -
Characterisation of a second gain of function EDAR variant, encoding EDAR380R, in East Asia
Bash scripts, R scripts, rehh output data. -
Comparative profiling of murine CLN1 Thalamus and Cortex at early and late disease timepoints
CLN1 disease or Infantile neuronal ceroid lipofuscinosis is a fatal inherited neurodegenerative lysosomal storage disease of early childhood with no currently available therapy. CLN1 disease is caused by mutations in the ... -
Construction of OOEP_IVT, OOEP_CAG, OOEP_VASA and ROSA26_gRNAs plasmids
These pdf files outline the construction of the four CRISPR/Cas9 gene drive DNA plasmid constructs developed during Gus McFarlane's PhD (2016 to 2020). These files provide important supplementary information to his PhD ... -
Single cell transcriptional profiling of rat embryonic stem cells defined by Rex1-EGFP reporter expression
Rat embryonic stem cells exhibit basal levels of spontaneous differentiation in 2i+LIF (2iL) culture. Expression of a stem cell specific Rex1-EGFP knock-in reporter transgene in rat ESCs was used to isolate functionally ... -
Proteomic profiling of primate synapses during normal healthy ageing
Normal mammalian brain ageing is characterised by the selective loss of discrete populations of dendritic spines and synapses, particularly affecting neuroanatomical regions such as the hippocampus. Although previous ... -
Investigating early cellular and molecular responses to misfolded protein
PMDs such as AD, PD, HD and TSE are associated with the accumulation of abnormally folded proteins. However, the mechanisms involving seeding, processing and degradation of these rogue proteins are poorly understood. In ... -
SUPERSEDED - Synaptic and non synaptic proteome in ageing murine cortical isolates
## SUPERSEDED - this dataset has been replaced by the one which can be found at https://doi.org/10.7488/ds/3181 . ## Label free proteomic analysis of "synaptic" and "non-synaptic" mitochondrial subpopulations isolated ... -
Proteomic analysis for peripherally accessible biomarkers in Neuronal Ceroid Lipofuscinosis (NCL)
iTRAQ and Label free Proteomic data generated from Skeletal muscle of CLN3 and PPT1 murine models of Batten disease. -
TMT generated proteomic identification and quantification of muscle and NMJ enriched fractions in mice and humans
The neuromuscular junction (NMJ) plays a fundamental role in transferring information from lower motor neurons to skeletal muscle to generate movement. It is also an experimentally-accessible model synapse routinely studied ... -
Somatic Cell Nuclear Transfer (Cloning) Efficiency
Cloning by nuclear transfer from adult somatic cells is a remarkable demonstration of developmental plasticity. When a nucleus is placed in oocyte cytoplasm, the changes in chromatin structure that govern differentiation ... -
Identification of miRNAs associated with the follicular-luteal transition in the ruminant ovary
(University of Edinburgh. Roslin Institute, 2013-03-26)Little is known about the involvement of microRNAs (miRNAs) in the follicular-luteal transition. The aim of this study was to identify genome-wide changes in miRNAs associated with follicular differentiation in sheep. miRNA ...
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