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Depositordc.contributorCaughey, Sarah
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherScottish Governmenten_UK
Spatial Coveragedc.coverage.spatialUKen
Spatial Coveragedc.coverage.spatialUNITED KINGDOMen
Time Perioddc.coverage.temporalstart=2015-03; end=2017-09; scheme=W3C-DTFen
Data Creatordc.creatorCaughey, Sarah
Data Creatordc.creatorDunn, Ian
Data Creatordc.creatorWilson, Peter
Data Creatordc.creatorReid, Angus
Data Creatordc.creatorBoswell, Timothy
Data Creatordc.creatorMukhtar, Nasir
Data Creatordc.creatorBrocklehurst, Sarah
Data Creatordc.creatorD'Eath, Rick
Citationdc.identifier.citationCaughey, Sarah; Dunn, Ian; Wilson, Peter; Reid, Angus; Boswell, Timothy; Mukhtar, Nasir; Brocklehurst, Sarah; D'Eath, Rick. (2018). Sex, diet density and feeding centre gene expression, 2015-2017 [dataset]. University of Edinburgh. Roslin Institute.
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Dataset Description (abstract)dc.description.abstract# Abstract # ## Background ## Research into energy balance and growth has infrequently considered genetic sex, yet there is sexual dimorphism for growth across the animal kingdom. We test the hypothesis that sexual dimorphism for growth and body weight in birds is potentially dependent on hypothalamic agouti-related peptide (AGRP) expression, since previous research indicates hypothalamic AGRP expression is correlated with growth potential. Because growth has been heavily selected in some chicken lines, food restriction is necessary to improve reproductive performance and welfare, but this increases hunger. Dietary dilution has been proposed to ameliorate this undesirable effect. We aimed to distinguish the effects of gut fullness from nutritional feedback on hypothalamic gene expression and its interaction with sex. ## Methods ## Restricted-fed 12-week-old male and female fast growing chickens were released from restriction and fed ad-libitum or a diet diluted with a non-nutritive bulking agent, ispaghula husk, for 2 days. A control group remained on the quantitative restricted diet. The basal hypothalamus was then dissected and gene expression of hypothalamic satiety peptides were measured using real-time PCR. To confirm observed sex difference effects, the experiment was repeated using only ad-libitum and restricted fed broiler breeders and observations made in an unrelated breed of ad-libitum fed male and female chickens. Linear Mixed Models (Genstat 18) were used for statistical analysis with transformation where appropriate. ## Results ## There were pronounced sex differences in a fast growing chicken strain, AGRP (P<0.001) expression was higher in males. In genetically distinct chickens, males had higher AGRP mRNA (P=0.002) expression than females, suggesting sex difference was not restricted to a fast growing strain. AGRP (P<0.001) expression was significantly decreased in ad-libitum-fed birds but was high and indistinguishable between birds on a quantitative versus qualitative restricted diet. Inversely, anorectic expression was significantly higher in ad-libitum fed birds. ## Conclusion ## Expression of orexigenic peptides in the avian hypothalamus are correlated with sex differences in growth and body weight. The differences in gene expression between sexes provides further evidence of AGRP expression being correlated to growth potential. Results also suggest that gut-fill alone does not reduce orexigenic gene expression.en_UK
Publisherdc.publisherUniversity of Edinburgh. Roslin Instituteen_UK
Relation (Is Referenced By)dc.relation.isreferencedby
Relation (Is Referenced By)dc.relation.isreferencedbyCaughey, S., Wilson, P., Mukhtar, N., Brocklehurst, S., Reid, A., D'Eath, R., Boswell, T., & Dunn, I. (2018). Sex differences in basal hypothalamic anorectic and orexigenic gene expression and the effect of quantitative and qualitative food restriction. Biology of Sex Differences, 9(1), [20].
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectBody Weight
Subject Classificationdc.subject.classificationVeterinary Sciences Agriculture and related subjectsen_UK
Titledc.titleSex, diet density and feeding centre gene expressionen_UK

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