Experimental data for the article "Predictable Properties of Fitness Landscapes Induced by Adaptational Tradeoffs"

Abstract

We used strains from Marcusson et al. (2009) (courtesy of Douglas Huseby and Diarmaid Hughes). The strains are isogenic derivatives of MG1655, a K12 strain of the bacterium E. coli, with specific point mutations or gene deletions in five different loci: gyrA:S83L, gyrA:D87N, parC:S80I, ΔmarR, and ΔacrR. There are 32 possible combinations of these alleles, but we only used the wild type, single mutants (5 strains) and double mutants (8 strains of 10 possible combinations): LM179 (00000), LM378 (10000), LM534 (01000), LM792 (00100), LM202 (00010), LM351 (00001), LM625 (11000), LM862 (10100), LM421 (10010), LM647 (10001), LM1124 (01100), LM538 (01010), LM592 (01001), LM367 (00011). A binary sequence after the strain’s name represents the presence/absence of a particular mutated allele (order as in the above list of genetic alterations).

Reference: Marcusson, Linda L., Niels Frimodt-Møller, and Diarmaid Hughes. "Interplay in the selection of fluoroquinolone resistance and bacterial fitness." PLoS pathogens 5.8 (2009).