Waclaw, Bartlomiej; Direito, Susana; Das, Suman; Allen, Rosalind; Krug, Joachim. (2020). Experimental data for the article "Predictable Properties of Fitness Landscapes Induced by Adaptational Tradeoffs", [dataset]. University of Edinburgh. School of Physics and Astronomy. https://doi.org/10.7488/ds/2756.
We used strains from Marcusson et al. (2009) (courtesy of Douglas Huseby and Diarmaid Hughes). The strains are isogenic derivatives of MG1655, a K12 strain of the bacterium E. coli, with specific point mutations or gene deletions in five different loci: gyrA:S83L, gyrA:D87N, parC:S80I, ΔmarR, and ΔacrR. There are 32 possible combinations of these alleles, but we only used the wild type, single mutants (5 strains) and double mutants (8 strains of 10 possible combinations): LM179 (00000), LM378 (10000), LM534 (01000), LM792 (00100), LM202 (00010), LM351 (00001), LM625 (11000), LM862 (10100), LM421 (10010), LM647 (10001), LM1124 (01100), LM538 (01010), LM592 (01001), LM367 (00011). A binary sequence after the strain’s name represents the presence/absence of a particular mutated allele (order as in the above list of genetic alterations).
Reference: Marcusson, Linda L., Niels Frimodt-Møller, and Diarmaid Hughes. "Interplay in the selection of fluoroquinolone resistance and bacterial fitness." PLoS pathogens 5.8 (2009).
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