LIVE-PAINT Supplementary Videos
Date Available
2020-04-07Type
datasetData Creator
Oi, CurranHorrocks, Mathew
Regan, Lynne
Publisher
University of Edinburgh. Quantitative Biology, Biochemistry and BiotechnologyRelation (Is Version Of)
https://doi.org/10.7488/ds/2839Relation (Is Referenced By)
https://doi.org/10.1101/2020.02.03.932228Metadata
Show full item recordAltmetric
Citation
Oi, Curran; Horrocks, Mathew; Regan, Lynne. (2020). LIVE-PAINT Supplementary Videos, [dataset]. University of Edinburgh. Quantitative Biology, Biochemistry and Biotechnology. https://doi.org/10.7488/ds/2801.Description
We present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extended data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein. Please note, compressed versions of the mp4 files are available at https://doi.org/10.7488/ds/2839 .The following licence files are associated with this item: