Millar, Andrew; Wenden, Benedicte; Hodge, Sarah. (2021). Spatiotemporal clock patterns in the leaf blade, [dataset]. University of Edinburgh. School of Biological Sciences. SynthSys.. https://doi.org/10.7488/ds/3030.
We used luciferase reporter gene imaging to monitor circadian rhythms in leaves of Arabidopsis thaliana plants, achieving resolution close to the cellular level. Leaves grown without environmental cycles for up to 3 wk reproducibly showed spatiotemporal waves of gene expression consistent with intercellular coupling, using several reporter genes.
This dataset contains:
1) Experiments performed by Benedicte Wenden:
- CCA1_LDgrownLLimaged_180310 - CCA1_LL_040210
- CCA1_LL_251109 - CCR2_LL_300610 - TOC1_LL_140710
2) Experiments performed by Sarah Hodge after the publication of Wenden et al., 2012:
The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336, VAT Registration Number GB 592 9507 00, and is acknowledged by the UK authorities as a “Recognised body” which has been granted degree awarding powers.