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Depositordc.contributorHindle, Matthew
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Data Creatordc.creatorMartin, Sarah F
Data Creatordc.creatorNoordally, Zeenat B
Data Creatordc.creatorvan Ooijen, Gerben
Data Creatordc.creatorBarrios-Llerena, Martin E
Data Creatordc.creatorSimpson, T Ian
Data Creatordc.creatorMillar, Andrew J
Data Creatordc.creatorHindle, Matthew M
Data Creatordc.creatorLe Bihan, Thierry
Date Accessioneddc.date.accessioned2014-06-24T15:10:14Z
Date Availabledc.date.available2014-06-24T15:10:14Z
Date Issueddc.date.issued2014-06-24
Citationdc.identifier.citationMartin, Sarah F.; Noordally, Zeenat B.; van Ooijen, Gerben; Barrios-Llerena, Martin E.; Simpson, T. Ian; Millar, Andrew J.; Hindle, Matthew M.; Thierry Le Bihan. (2014). The reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model species, [Dataset]. University of Edinburgh. SynthSys and School of Biological Sciences. https://doi.org/10.7488/ds/72.en
Persistent Identifierdc.identifier.urihttps://hdl.handle.net/10283/563
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/72
Dataset Description (abstract)dc.description.abstract## Background ## The current knowledge of eukaryote signalling originates from phenotypically diverse organisms. There is a pressing need to identify conserved signalling components among eukaryotes, which will lead to the transfer of knowledge across kingdoms. Two useful properties of a eukaryote model for signalling are (1) reduced signalling complexity, (2) conservation of signalling components. The alga Ostreococcus tauri is described as the smallest free-living eukaryote. With less than 8,000 genes, it represents a highly constrained genomic palette. ## Results ## Our survey revealed 133 protein kinases and 34 protein phosphatases (1.7% and 0.4% of the proteome). We conducted phospho-proteomic experiments and constructed domain structures and phylogenies for the catalytic protein-kinases. For each of the major kinases families we review the completeness and divergence of O. tauri representatives in comparison to the well-studied kinomes of the laboratory models Arabidopsis thaliana and Saccharomyces cerevisiae, and of Homo sapiens. Many kinase clades in O. tauri were reduced to a single member, in preference to the loss of family diversity, whereas TKL and ABC1 clades were expanded. We also identified kinases that have been lost in A. thaliana but retained in O. tauri. For three, contrasting eukaryotic pathways – TOR, MAPK, and the circadian clock – we established the subset of conserved components and demonstrate conserved sites of substrate phosphorylation and kinase motifs. ## Conclusions ## We conclude that O. tauri satisfies our two central requirements. Several of its kinases are more closely related to H. sapiens orthologs than S. cerevisiae is to H. sapiens. The greatly reduced kinome of O. tauri is therefore a suitable model for signalling in free-living eukaryotes.en_UK
Publisherdc.publisherUniversity of Edinburgh. SynthSys and School of Biological Sciencesen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyHindle et al. (2014) "The reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model species" BMC Genomics 2014, 15:640. http://www.biomedcentral.com/1471-2164/15/640
Subjectdc.subjectconserved eukaryote signallingen_UK
Subjectdc.subjectprotein kinase phylogenyen_UK
Subjectdc.subjectOstreococcus taurien_UK
Subjectdc.subjectTOR signallingen_UK
Subjectdc.subjectMAPK cascadeen_UK
Subjectdc.subjectcircadian clocken_UK
Subjectdc.subjectphosphorylationen_UK
Subjectdc.subjectmodel kinomeen_UK
Subject Classificationdc.subject.classificationBiological Sciences::Biologyen_UK
Titledc.titleThe reduced kinome of Ostreococcus tauri: core eukaryotic signalling components in a tractable model speciesen_UK
Typedc.typedataseten_UK

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