Construction of a modular arsenic resistance operon in E. coli and the production of arsenic nanoparticles: Figure 6 original images

Abstract

Original scanned images of pArs deletion mutants in E. coli on arsenic and 0.1mM IPTG-containing agar plates

Panel A; Image 1: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+C3; pArs_lac ΔEC20; 0mM, 5mM sodium arsenate; 24hr incubation Panel A; Image 2: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 10mM, 20mM sodium arsenate; 24hr incubation Panel A; Image 3: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 30mM sodium arsenate; 24hr incubation Panel A; Image 4: pEC20 & pArsRBCC; 0mM, 5mM, 10mM, 20mM, 30mM sodium arsenate; 24hr incubation

Panel B; Image 1: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 0mM, 5mM sodium arsenate; 3 day incubation Panel B; Image 2: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 10mM, 20mM sodium arsenate; 3 day incubation Panel B; Image 3: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 30mM, 40mM As; 3 day incubation Panel B; Image 4: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 50mM, 60mM As; 3 day incubation Panel B; Image 5: 0.1 mM IPTG; pET28b, pArs_lac; pArs_lac ΔC2; pArs_lac ΔC3; pArs_lac ΔC2+ΔC3; pArs_lac ΔEC20; 70mM, 80mM As; 3 day incubation Panel B; Image 6: pEC20 & pArsRBCC; 0mM, 5mM, 10mM, 20mM, 30mM As; 3 day incubation Panel B; Image 7: pEC20 & pArsRBCC; 40mM, 50mM, 60mM, 70mM, 80mM As; 3 day incubation